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kits for bio-plex phosphoprotein detection/lysis  (Bio-Rad)


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    Bio-Rad kits for bio-plex phosphoprotein detection/lysis
    (A) Cells left untreated (co), stimulated with NGF (25 ng/ml) or alternatively with NGF (25 ng/ml) and PD098059 (50 µM) for 16 h and were stained with Phalloidin-TRITC and Hoechst 33342. Pictures were taken of 3–4 independent microscopic fields and are representative of 3 experiments. Scale bar = 20 µm. (B, C) PC12 cells were stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml) and activation of p42/44 MAPK was measured by <t>Bio-Plex</t> <t>phosphoprotein</t> <t>detection</t> assay at various time points (0 min –20 min). Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 (B). Total cell lysates were analyzed with western blotting done in parallel for 0 and 5 min. (D, E) PC12 cells were transfected with control or specific siRNA for MAPK. After 72 h in culture, cells were stimulated with NGF (5 ng/ml). Additional 3 d pictures were taken of 3–5 microscopic fields (D) and neurite-bearing cells are expressed as fold of control (f.o.c.; control value was 2.16±0.53% neurite-bearing cells) in (E). Pictures are representative of 5 independent experiments. Values represent the mean ± SEM, n = 5. Scale bar = 20 µm. Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01.
    Kits For Bio Plex Phosphoprotein Detection/Lysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 2043 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kits for bio-plex phosphoprotein detection/lysis/product/Bio-Rad
    Average 90 stars, based on 2043 article reviews
    kits for bio-plex phosphoprotein detection/lysis - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "LAMTOR2-Mediated Modulation of NGF/MAPK Activation Kinetics during Differentiation of PC12 Cells"

    Article Title: LAMTOR2-Mediated Modulation of NGF/MAPK Activation Kinetics during Differentiation of PC12 Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0095863

    (A) Cells left untreated (co), stimulated with NGF (25 ng/ml) or alternatively with NGF (25 ng/ml) and PD098059 (50 µM) for 16 h and were stained with Phalloidin-TRITC and Hoechst 33342. Pictures were taken of 3–4 independent microscopic fields and are representative of 3 experiments. Scale bar = 20 µm. (B, C) PC12 cells were stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml) and activation of p42/44 MAPK was measured by Bio-Plex phosphoprotein detection assay at various time points (0 min –20 min). Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 (B). Total cell lysates were analyzed with western blotting done in parallel for 0 and 5 min. (D, E) PC12 cells were transfected with control or specific siRNA for MAPK. After 72 h in culture, cells were stimulated with NGF (5 ng/ml). Additional 3 d pictures were taken of 3–5 microscopic fields (D) and neurite-bearing cells are expressed as fold of control (f.o.c.; control value was 2.16±0.53% neurite-bearing cells) in (E). Pictures are representative of 5 independent experiments. Values represent the mean ± SEM, n = 5. Scale bar = 20 µm. Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01.
    Figure Legend Snippet: (A) Cells left untreated (co), stimulated with NGF (25 ng/ml) or alternatively with NGF (25 ng/ml) and PD098059 (50 µM) for 16 h and were stained with Phalloidin-TRITC and Hoechst 33342. Pictures were taken of 3–4 independent microscopic fields and are representative of 3 experiments. Scale bar = 20 µm. (B, C) PC12 cells were stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml) and activation of p42/44 MAPK was measured by Bio-Plex phosphoprotein detection assay at various time points (0 min –20 min). Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 (B). Total cell lysates were analyzed with western blotting done in parallel for 0 and 5 min. (D, E) PC12 cells were transfected with control or specific siRNA for MAPK. After 72 h in culture, cells were stimulated with NGF (5 ng/ml). Additional 3 d pictures were taken of 3–5 microscopic fields (D) and neurite-bearing cells are expressed as fold of control (f.o.c.; control value was 2.16±0.53% neurite-bearing cells) in (E). Pictures are representative of 5 independent experiments. Values represent the mean ± SEM, n = 5. Scale bar = 20 µm. Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01.

    Techniques Used: Staining, Activation Assay, Detection Assay, Western Blot, Transfection

    (A, B) PC12 cells were transfected with control or specific siRNA for LAMTOR2. After 24 h, cells were left untreated (co) or stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml). After various time points (0, 2, 5, 10, 20 min), activation of p42/44 MAPK was measured with the Bio-Plex phosphoprotein detection assay. Values represent the mean ± SEM, n = 3. Differences were analyzed using unpaired two-tailed t-test: ***p<0.001. (B) In parallel, after 5 min, total cell lysates were analyzed by western blotting technique. (C) Cells were transfected with LAMTOR2 siRNA plus control vector or plus a human LAMTOR2 ortholog. After stimulation with NGF (25 ng/ml) for 5 min Bio-Plex phosphoprotein detection assay was carried out and p42/44 MAPK activation expressed as fold of control (f.o.c. control value was 4.57±0.67%). Values represent the mean ± SEM, n = 7. Differences were analyzed using unpaired two-tailed t-test: *p<0.05.
    Figure Legend Snippet: (A, B) PC12 cells were transfected with control or specific siRNA for LAMTOR2. After 24 h, cells were left untreated (co) or stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml). After various time points (0, 2, 5, 10, 20 min), activation of p42/44 MAPK was measured with the Bio-Plex phosphoprotein detection assay. Values represent the mean ± SEM, n = 3. Differences were analyzed using unpaired two-tailed t-test: ***p<0.001. (B) In parallel, after 5 min, total cell lysates were analyzed by western blotting technique. (C) Cells were transfected with LAMTOR2 siRNA plus control vector or plus a human LAMTOR2 ortholog. After stimulation with NGF (25 ng/ml) for 5 min Bio-Plex phosphoprotein detection assay was carried out and p42/44 MAPK activation expressed as fold of control (f.o.c. control value was 4.57±0.67%). Values represent the mean ± SEM, n = 7. Differences were analyzed using unpaired two-tailed t-test: *p<0.05.

    Techniques Used: Transfection, Activation Assay, Detection Assay, Two Tailed Test, Western Blot, Plasmid Preparation



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    (A) Cells left untreated (co), stimulated with NGF (25 ng/ml) or alternatively with NGF (25 ng/ml) and PD098059 (50 µM) for 16 h and were stained with Phalloidin-TRITC and Hoechst 33342. Pictures were taken of 3–4 independent microscopic fields and are representative of 3 experiments. Scale bar = 20 µm. (B, C) PC12 cells were stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml) and activation of p42/44 MAPK was measured by <t>Bio-Plex</t> <t>phosphoprotein</t> <t>detection</t> assay at various time points (0 min –20 min). Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 (B). Total cell lysates were analyzed with western blotting done in parallel for 0 and 5 min. (D, E) PC12 cells were transfected with control or specific siRNA for MAPK. After 72 h in culture, cells were stimulated with NGF (5 ng/ml). Additional 3 d pictures were taken of 3–5 microscopic fields (D) and neurite-bearing cells are expressed as fold of control (f.o.c.; control value was 2.16±0.53% neurite-bearing cells) in (E). Pictures are representative of 5 independent experiments. Values represent the mean ± SEM, n = 5. Scale bar = 20 µm. Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01.
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    (A) Cells left untreated (co), stimulated with NGF (25 ng/ml) or alternatively with NGF (25 ng/ml) and PD098059 (50 µM) for 16 h and were stained with Phalloidin-TRITC and Hoechst 33342. Pictures were taken of 3–4 independent microscopic fields and are representative of 3 experiments. Scale bar = 20 µm. (B, C) PC12 cells were stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml) and activation of p42/44 MAPK was measured by <t>Bio-Plex</t> <t>phosphoprotein</t> <t>detection</t> assay at various time points (0 min –20 min). Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 (B). Total cell lysates were analyzed with western blotting done in parallel for 0 and 5 min. (D, E) PC12 cells were transfected with control or specific siRNA for MAPK. After 72 h in culture, cells were stimulated with NGF (5 ng/ml). Additional 3 d pictures were taken of 3–5 microscopic fields (D) and neurite-bearing cells are expressed as fold of control (f.o.c.; control value was 2.16±0.53% neurite-bearing cells) in (E). Pictures are representative of 5 independent experiments. Values represent the mean ± SEM, n = 5. Scale bar = 20 µm. Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01.
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    (A) Cells left untreated (co), stimulated with NGF (25 ng/ml) or alternatively with NGF (25 ng/ml) and PD098059 (50 µM) for 16 h and were stained with Phalloidin-TRITC and Hoechst 33342. Pictures were taken of 3–4 independent microscopic fields and are representative of 3 experiments. Scale bar = 20 µm. (B, C) PC12 cells were stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml) and activation of p42/44 MAPK was measured by <t>Bio-Plex</t> <t>phosphoprotein</t> <t>detection</t> assay at various time points (0 min –20 min). Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 (B). Total cell lysates were analyzed with western blotting done in parallel for 0 and 5 min. (D, E) PC12 cells were transfected with control or specific siRNA for MAPK. After 72 h in culture, cells were stimulated with NGF (5 ng/ml). Additional 3 d pictures were taken of 3–5 microscopic fields (D) and neurite-bearing cells are expressed as fold of control (f.o.c.; control value was 2.16±0.53% neurite-bearing cells) in (E). Pictures are representative of 5 independent experiments. Values represent the mean ± SEM, n = 5. Scale bar = 20 µm. Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01.
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    Image Search Results


    (A) Cells left untreated (co), stimulated with NGF (25 ng/ml) or alternatively with NGF (25 ng/ml) and PD098059 (50 µM) for 16 h and were stained with Phalloidin-TRITC and Hoechst 33342. Pictures were taken of 3–4 independent microscopic fields and are representative of 3 experiments. Scale bar = 20 µm. (B, C) PC12 cells were stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml) and activation of p42/44 MAPK was measured by Bio-Plex phosphoprotein detection assay at various time points (0 min –20 min). Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 (B). Total cell lysates were analyzed with western blotting done in parallel for 0 and 5 min. (D, E) PC12 cells were transfected with control or specific siRNA for MAPK. After 72 h in culture, cells were stimulated with NGF (5 ng/ml). Additional 3 d pictures were taken of 3–5 microscopic fields (D) and neurite-bearing cells are expressed as fold of control (f.o.c.; control value was 2.16±0.53% neurite-bearing cells) in (E). Pictures are representative of 5 independent experiments. Values represent the mean ± SEM, n = 5. Scale bar = 20 µm. Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01.

    Journal: PLoS ONE

    Article Title: LAMTOR2-Mediated Modulation of NGF/MAPK Activation Kinetics during Differentiation of PC12 Cells

    doi: 10.1371/journal.pone.0095863

    Figure Lengend Snippet: (A) Cells left untreated (co), stimulated with NGF (25 ng/ml) or alternatively with NGF (25 ng/ml) and PD098059 (50 µM) for 16 h and were stained with Phalloidin-TRITC and Hoechst 33342. Pictures were taken of 3–4 independent microscopic fields and are representative of 3 experiments. Scale bar = 20 µm. (B, C) PC12 cells were stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml) and activation of p42/44 MAPK was measured by Bio-Plex phosphoprotein detection assay at various time points (0 min –20 min). Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 (B). Total cell lysates were analyzed with western blotting done in parallel for 0 and 5 min. (D, E) PC12 cells were transfected with control or specific siRNA for MAPK. After 72 h in culture, cells were stimulated with NGF (5 ng/ml). Additional 3 d pictures were taken of 3–5 microscopic fields (D) and neurite-bearing cells are expressed as fold of control (f.o.c.; control value was 2.16±0.53% neurite-bearing cells) in (E). Pictures are representative of 5 independent experiments. Values represent the mean ± SEM, n = 5. Scale bar = 20 µm. Differences were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test: *p<0.05, **p<0.01.

    Article Snippet: Kits for Bio-Plex phosphoprotein detection/lysis were obtained from Biorad (Vienna, Austria).

    Techniques: Staining, Activation Assay, Detection Assay, Western Blot, Transfection

    (A, B) PC12 cells were transfected with control or specific siRNA for LAMTOR2. After 24 h, cells were left untreated (co) or stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml). After various time points (0, 2, 5, 10, 20 min), activation of p42/44 MAPK was measured with the Bio-Plex phosphoprotein detection assay. Values represent the mean ± SEM, n = 3. Differences were analyzed using unpaired two-tailed t-test: ***p<0.001. (B) In parallel, after 5 min, total cell lysates were analyzed by western blotting technique. (C) Cells were transfected with LAMTOR2 siRNA plus control vector or plus a human LAMTOR2 ortholog. After stimulation with NGF (25 ng/ml) for 5 min Bio-Plex phosphoprotein detection assay was carried out and p42/44 MAPK activation expressed as fold of control (f.o.c. control value was 4.57±0.67%). Values represent the mean ± SEM, n = 7. Differences were analyzed using unpaired two-tailed t-test: *p<0.05.

    Journal: PLoS ONE

    Article Title: LAMTOR2-Mediated Modulation of NGF/MAPK Activation Kinetics during Differentiation of PC12 Cells

    doi: 10.1371/journal.pone.0095863

    Figure Lengend Snippet: (A, B) PC12 cells were transfected with control or specific siRNA for LAMTOR2. After 24 h, cells were left untreated (co) or stimulated with either NGF (25 ng/ml) or EGF (100 ng/ml). After various time points (0, 2, 5, 10, 20 min), activation of p42/44 MAPK was measured with the Bio-Plex phosphoprotein detection assay. Values represent the mean ± SEM, n = 3. Differences were analyzed using unpaired two-tailed t-test: ***p<0.001. (B) In parallel, after 5 min, total cell lysates were analyzed by western blotting technique. (C) Cells were transfected with LAMTOR2 siRNA plus control vector or plus a human LAMTOR2 ortholog. After stimulation with NGF (25 ng/ml) for 5 min Bio-Plex phosphoprotein detection assay was carried out and p42/44 MAPK activation expressed as fold of control (f.o.c. control value was 4.57±0.67%). Values represent the mean ± SEM, n = 7. Differences were analyzed using unpaired two-tailed t-test: *p<0.05.

    Article Snippet: Kits for Bio-Plex phosphoprotein detection/lysis were obtained from Biorad (Vienna, Austria).

    Techniques: Transfection, Activation Assay, Detection Assay, Two Tailed Test, Western Blot, Plasmid Preparation